Application

Fluorescein di(β-D-galactopyranoside) (FDG) has been used for the detection of gene expression via FACS (fluorescence-activated cell sorting).

Fluorescein di(β-D-galactopyranoside) has been used as a substrate in β-galactosidase assay.

Biochem/physiol Actions

Fluorescein di (β-D-galactopyranoside) (FDG) results in fluorescence after being hydrolyzed to fluorescein by β-galactosidase. It gives fluorescence proportional to the enzymatic activity. This molecule is mainly required for the detection of gene expression in transfected cells when using β-galactosidase (Lac Z) as a reporter gene.

Packaging

1, 5, 25 mg in glass insert

Substrates

Fluorogenic substrate for β-galactosidase