Application

Formaldehyde Dehydrogenase from Pseudomonas sp. has been used in standard curve generation for the demethylation-FDH assay of lysine specific demethylase 1 (LSD1) and in formaldehyde dehydrogenase (FDH) assay.

Formaldehyde dehydrogenase is used as a biosensor for the presence of formaldehyde in pharmaceuticals, waste water, vaccines and industrial products. It was also used in coupled pectin methyl esterase (PME) enzyme assay.

Biochem/physiol Actions

Formaldehyde dehydrogenases elicit protection from formaldehyde and are regarded as detoxification system.

Formaldehyde dehydrogenase catalyzes the conversion of formaldehyde to formate.

General description

Formaldehyde Dehydrogenase (FDH) belongs to the medium-chain alcohol dehydrogenase family. It is encoded by the fdhA and fdhB gene in Pseudomonas aeruginosa. The FDH sequences of P. aeruginosa and P. putida are conserved and show homology. FDH exists as dimer and tetramer with zinc in their catalytic active site. The monomer corresponds to 350-400 amino-acid residues.

Packaging

5, 25, 50 units in glass bottle

Physical form

Lyophilized powder containing ~70% stabilizers as Mg²⁺, Ca²⁺, bovine serum albumin, glycine, and lysine

Unit Definition

One unit will oxidize 1.0 µmole of formaldehyde to formic acid per min at pH 7.5 at 37 °C.