IMAC SepFast BG is specifically designed for batch or gravity flow purifications of Histidine-tagged proteins. The SepFast rough surface
and carefully controlled pore structure allows fast access of the tagged protein molecules to the immobilised metal ligands. IMAC
SepFast BG also benefits from high mechanical strength which permits liquid passing through gravity columns at optimal flow rates.
Clarified or unclarified cell lysates and culture broths can be directly processed. All these translate into high process flexibility and higher
protein yield at shortened purification time. IMAC SepFast BG or its four metal charged formats i.e. Ni, Cu, Co and Zn are highly stable
and compatible with a wide range of detergents, denaturing reagents and reducing agents that are commonly used for protein
purification protocols. A screening kit containing all four types of immobilised metal ions is also available for determination of the optimal
metal affinity for a given protein.
- Single column purification (batch or gravity flow)
- High binding capacity ( >40mg/ml) and high sample purity
- Virtually no metal leaching
- Includes 2 gravity flow columns for scalable use of resin volume