Application

Anti-FOXA1 antibody produced in rabbit has been used in immunoblotting.

Anti-FOXA1 antibody produced in rabbit is suitable for indirect immunofluorescence at a dilution of 1:100-1:200 using MCF7 cells fixed with paraformaldehyde-Triton. It is also suitable for western blotting at a dilution of 1:250-1:500 using extracts of the MCF7 cell line.

Biochem/physiol Actions

Forkhead box protein A1 (FOXA1) exhibits the greatest extent of mitotic chromosome binding. FOXA1 is involved in embryonic development and cell differentiation. It promotes cell proliferation by androgen receptor (AR) and activates Notch pathway. FOXA1 acts as a negative regulator in the initial stages of lung cancer metastasis. It may modulate the epithelial-to-mesenchymal transition through its transcriptional regulatory network. FOXA1 has been implicated in certain tumor types including breast, prostate, lung, thyroid and esophageal squamous cell carcinomas. It might act as a potential regulator of progression of human glioma.

FOXA group of proteins are referred to as “pioneer factors” as they interact with compacted chromatin and make them accessible for transcription factors. FOXA1 is a crucial partner in events that lead to breast cancer progression in connection with estrogen receptor (ER).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Forkhead box protein A1 (FOXA1) is a protein encoded by the FOXA1 gene in humans and is a member of the FOX family of transcription factors.

FOXA1 gene is mapped to human chromosome 14q21.1. FOXA1 belongs to the FOXA family. Forkhead proteins comprise “winged helix” DNA binding or forkhead domain.

Immunogen

synthetic peptide corresponding to amino acids 441-459 of human FOXA1, conjugated to KLH via a N-terminal cysteine residue.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Anti-FOXA1 specifically recognizes human FOXA1.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.