Application

When performing small scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview^™ Red Affinity Gels greatly reduces the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Small-scale affinity capture of fusion recombinant proteins with His-tags / histidine-containing tags (His-tagged proteins).

Features and Benefits

Increased visibility - Red color reduces risk of incidental aspiration Improved recovery of target protein by reduced accidental loss Higher reproducibility - More consistent yields

Legal Information

HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC

EZview is a trademark of Sigma-Aldrich Co. LLC

Packaging

1 mL in poly bottle

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon