Application

Anti-AtEB1b (C-terminal) antibody produced in rabbit is suitable for western blotting at a concentration of 1-2µg/mL using leaves extract (cytosolic fraction) of transgenic Arabidopsis seedlings expressing YFP-tagged AtEB1b.

Biochem/physiol Actions

End-binding 1 (EB1) proteins are plus-end-tracking proteins that are specifically localized to growing microtubule plus ends that regulate microtubule and interact with intracellular targets. The plant-specific EB1 subtype functions on the spindle microtubules by accumulating in the prophase nucleus. The microtubule-end-binding protein expressed in Arabidopsis thaliana cells (is called as AtEB1a) is fused with green fluorescent protein (GFP) resulting in labelling of spindle poles. During interphase, AtEB1a-GFP labels the microtubule plus end and also marks the minus end. The microtubule associated protein END BINDING 1b (EB1b) is involved as mutants and dampens root responses to touch and enhances gravitropism. In animals, EB1 proteins have acidic C-terminal tails that might induce an auto-inhibitory conformation.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

synthetic peptide corresponding to amino acids 205-221 located in a region near the C-terminus of Arabidopsis AtEB1b, conjugated to KLH. This sequence is highly conserved (single amino acid substitution) in AtEB1a and AtEB1c.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Recognizes AtEB1b fusion protein expressed in transgenic Arabidopsis plants.