Application

ExtrAvidin^®-Peroxidase is suitable for the following applications: Dot blot at a minimum dilution of 1:4,000 Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100)Direct ELISA at a minimum dilution of 1:2,000It has also been used for:Immunoprecipitation and ImmunoblotingRa2I solid-phase binding assaysSouthwestern blotting Western blottingImmunocytochemistry

Biochem/physiol Actions

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin^® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Preparation Note

Affinity purified protein

Specificity

The product is found to be reactive with Anti-Avidin, Catalog Number A5170, and non-reactive with Anti-Streptavidin, Catalog Number S6390, by immunoelectrophoresis.

Storage and Stability

Store at 2-8 °C. Do Not Freeze. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.