Application

ExtrAvidin^™ -Alkaline Phosphatase has been used in:Dot blotImmunohistochemistry (formalin-fixed, paraffin-embedded sections) Indirect ELISA Enzyme linked immunosorbent assay (ELISA)Enzyme-linked immunosorbent spot (ELISPOT) analysisCompetitive ELISA

Biochem/physiol Actions

Alkaline Phosphatase catalyzes the hydrolysis of esters to yield phosphoric acid. It is also facilitate the transport of substances across membranes.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin^® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin has been conjugated to Alkaline Phosphatase and is used for various techniques.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Physical form

Solution in 0.05 M Tris-HCl buffer, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Affinity purified protein