Application

ELISA (1:5000-1:100,000) r>Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)

General description

This Goat Anti-Mouse IgG Alkaline Phosphatase Conjugate is validated for use in ELISA Immunoblotting for the detection of Mouse IgG.

Immunoaffinity purified goat polyclonal antibody adsorbed against human, bovine, and horse serum proteins and conjugated to alkaline phosphatase. Recognizes mouse IgG.

Immunogen

Mouse

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

To ensure the quality of these reagents each lot is tested with several of our primary antibodies. Antibody specificity is tested by immunoelectrophoresis and shown to be specific for mouse IgG and light chains common to most mouse immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins has been detected. The antibody has also been tested by ELISA and/or adsorbed to ensure minimal cross-reactivity with human, bovine, and horse serum proteins, but the antibody may exhibit cross-reactivity with immunoglobulins from other species. Antibody should be titrated for optimal results in individual systems.

Packaging

200 µg in Glass bottle

Physical form

Lyophilized from 250 mM NaCl, 10 mM Tris-HCl, 15 mg/ml BSA, pH 8.0.

Reconstitution

Reconstitute the antibody with 1 ml H₂O. Dissolve by gently mixing at 4°C, since extensive mixing will denature antibodies. Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Alternatively, glycerol (to a final concentration of 50%) may be added and the reconstituted antibody then stored at -20°C. Please note, under these conditions the concentration of the antibody and the buffer salts will be one-half of the original concentration.

Warning

Toxicity: Standard Handling (A)