Application

Denhardt′s Solution has been used:as a component in hybridization buffer for the hybridization of brain sectionsin in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISH_PAP-WM) processin the preparation of hybridization mixture

Suitable as a blocking Buffer for Southern and Northern blot membranes.

Biochem/physiol Actions

Denhardt′ssolutionand gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization.

Components

Each vial of Denhardt’;s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).

General description

Denhardt’;s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Packaging

5 mL in glass bottle

Preparation Note

To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at -20°C for up to two years.