Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Immunoglobulin G (IgG) is a glycoprotein antibody that modulates several immune responses. IgG activates the complement system and regulates the antibody-dependent cell-mediated cytotoxicity (ADCC).

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General description

Immunoglobulin G (IgG) constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classesi.eIgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains, two heavy chains (γchains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy and the light chains are linked at VH and CH₁ domain to form the Fab (fragment antigen binding) arm. The antigen binds to the V regions of theantibody.

Physical form

Rabbit Anti-Dog IgG is provided as a liquid containing 15 mM sodium azide as preservative.

Preparation Note

treated to remove lipoproteins

Specificity

The antiserum is determined to be immunospecific for dog IgG versus normal dog serum and dog IgG by immunoelectrophoresis. This antiserum has not been assayed for interspecies cross reactivity.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.