Application

The enzyme from Sigma has been used in the analysis of bile samples in various studies.

Biochem/physiol Actions

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg²⁺, Cu²⁺, and Cd²⁺) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.

Packaging

3000, 7500 units in poly bottle

1500 units in glass bottle

Physical form

Partially purified lyophilized powder containing buffer salts and stabilizer

Unit Definition

One unit will hydrolyze 1.0 µmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.