The SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first-strand cDNA synthesis, which displays excellent linearity across a wide range of starting material. This gives the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in qPCR studies.
SensiFAST™ cDNA Synthesis Kit contains a highly-pure reverse transcriptase and optimised TransAmp™ buffer system, which includes a unique blend of random hexamers and anchored oligo (dT) primers to deliver the highest quality qPCR ready cDNA. This makes the SensiFAST™ cDNA Synthesis Kit ideal for working with limited sample volumes, such as laser-micro dissected samples and tissue biopsies (down to 1 pg of input RNA), to reverse transcribe precious RNA into stable cDNA ready for accurate real-time quantification. The unique blend of random hexamer primers and anchored oligo dT in the TransAmp Buffer also ensure unbiased 3′and 5′coverage and reverse transcription of all regions.
SensiFAST™ cDNA Synthesis Kit can be used with SensiFAST™ Probe and SensiFAST™ SYBR® Kits for fast real-time RT-qPCR without compromising on quality, giving real-time results in less than an hour.
- Gene expression analysis
- Tissue biopsy analysis
- miRNA profiling / quantification
- RNA target detection
- Pathogen detection
This product has met the following criteria: