ACCUZYME™ is a thermostable enzyme possessing 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities, offering high fidelity. ACCUZYME provides high fidelity with an error-rate of 3.0 x 10-6, to give blunt-ended amplicons of up to 5Kb in length. ACCUZYME is supplied with 10x Reaction Buffer containing MgSO4, which provides optimal final reaction conditions (1.5mM Mg2+) for most experiments. In order to allow optimization of reaction conditions, additional MgCl2 is provided.
The specificity and performance of ACCUZYME can be further improved with the use of 2x PolyMate Additive (not included), which is designed for GC or AT-rich DNA, ""dirty"" templates or sequences with a high level of secondary structure.
- Efficient – highly productive target amplification and removal of 3’ A overhangs
- Accurate – possesses 3’ - 5’ proofreading exonuclease activity that delivers an error rate of 3.0 x 10-6 for increased PCR fidelity versus Taq DNA polymerase
- Sensitive – high-yield amplification from limiting amounts of human, animal and plant template DNA
- Robust – developed for reliable amplification of even the most challenging targets, including genomic DNA and GC-rich targets
- Flexible – ideal for amplifying any target up to 5 kb from DNA extracted from mammalian tissue samples
- Convenient – advanced buffering system minimizes the requirements for PCR optimization, thereby reducing time-to-results and eliminating the cost of unnecessary repeats
- Applications: High-fidelity PCR, routine cloning applications requiring increased PCR yield, blunt-end cloning, and site-directed mutagenesis
- Components: Accuzyme DNA Polymerase, 10x AccuBuffer, 50mM MgCl2 Solution
- Unit Definition: One unit will incorporate 10nmoles of dNTPs in 30min at 72°C
- Concentration: 2.5u/µl
- Storage Conditions: ACCUZYME can be stored at -20°C, full activity of the reagents is retained until the expiry date indicated on the outer box label, repeated freeze/thaw cycles should be avoided
This product has met the following criteria: