IMMOLASE™ is a heat-activated thermostable DNA polymerase. IMMOLASE provides extremely high yield and improved specificity when compared to standard polymerases and can eliminate the presence of non-specifics, such as primer-dimers and mis-primed products. IMMOLASE is inactive at room temperature and therefore prior to PCR cycling, requires activation by heat treatment for 10 minutes . This facilitates flexibility in reaction setup, including premixing of PCR reagents at room temperature. Subsequently, the reaction can be handled according to the user´s existing protocols for thermostable DNA polymerases. The specificity and performance of IMMOLASE can be further improved with the use of 2x PolyMate Additive (not supplied, see associated products), which is designed for GC- or AT-rich DNA, ""dirty"" templates or sequences with difficult melting profiles.
Features:
- Outstanding and robust performance
- For PCR assays requiring hot-start
- Convenient set up at room temperature
- Leaves ´A´ overhang
Applications:
- Ultra-high specificity for multiplex reactions
- TA cloning
Components:
IMMOLASE DNA Polymerase10x ImmoBuffer50mM MgCl2 SolutionUnit Definition: One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.Concentration: 5u/µl
Storage Conditions: IMMOLASE can be stored for 12 months at –20°C.
This product has met the following criteria: