Analysis Note

Positive ControlHuman endometrium tissue, HeLa cells

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Immunocytochemistry (10 g/ml)Paraffin Sections (0.7 g/ml)

General description

This Anti-NDRG1 Mouse mAb (2D7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of NDRG1.

Purified mouse monoclonal antibody. Recognizes the ~40-45 kDa NDRG1 protein.

Recognizes the ~40-45 kDa NDRG1 protein in HeLa cells and human endometrium tissue.

Immunogen

Full-length, recombinant human NDRG1 (aa 1-395), expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Jiang, K., et al. 2010. Scand. J. Gastroenterol45, 898.Leth-Larsen R, et al. 2009. Mol. Cell Proteomics8, 1436.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)