Application

Goat Anti-Human IgG Antibody, Fc, HRP conjugate has been used in enzyme linked immunosorbent assay (ELISA)(1:3,000). It is also suitable for use in: enzyme immunoassay (EIA) with OPD: 1:5,000-1:100,000 immunohistochemistry 1:500-1:5,000 western blot

Biochem/physiol Actions

Immunoglobulin G (IgG) is implicated inantibody-dependent cell-mediated cytotoxicity(ADCC). It aids in the activation of the classical pathway of the complement system.It neutralizes virus particles and toxins. Maternal IgG is transferred tothe fetusthrough the placenta that is crucial for protecting newborns againstinfectious diseases. Measurement of IgG levels in severe acute respiratory syndrome coronavirus (SARS‐CoV)‐2 infected patients can be used as a potential strategy to assess the severity and prognosis of coronavirus disease 2019 (COVID‐19).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG) is the most common antibody isotype found in the human serum. It exists in four isotypes IgG1, IgG2, IgG3, and IgG4. IgG structure is characterized with four polypeptide chains with two identical heavy (H) chains and two identical λ light (L) chains. These chains are inter-linked by disulfide bonds. IgG is produced by the B cells.

Immunogen

Human IgG

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.