Application

Research CategoryNeuroscience

Anti-Calbindin D-28K Antibody, Cat. No. ABN2192, is a highly specific rabbit polyclonal antibody that targets Calbindin D and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Calbindin D-28K in E18 rat cortical cells.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Calbindin D-28K in mouse kidney and human cerebellum tissue sections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Calbindin (UniProt: P12658; also known as Calbindin D28, D-28K, PCD-29, Spot 35 protein, Vitamin D-dependent calcium-binding protein, avian-type) is encoded by the calb1 gene (gene ID: 12307) in murine species. Calbindin D-28k is a member of a large family of intracellular calcium-binding proteins of the EF-hand related to calmodulin and troponin-C. Calbindin D-28K has four functional calcium-binding sites (aa 24-35; 111-122; 155-166; and 199-210). It has moderate cytoplasmic mobility and comprises six EF-hands, of which one or two are non-functioning in metal binding. EF-2 is viewed as non-metal binding and EF-6 may have a very low Ca2+ affinity. Four EF hands function in calcium binding with rapid to intermediate kinetics and affinity. The biological roles of Calbindin D-28K include calcium regulation and signaling, including calcium transport and uptake, calcification of bone and teeth, and calcium related signaling in neurons and transiently in embryological development. It also protects neurons from apoptotic cell death. Decline in Calbindin D-28K levels in hippocampal dentate granule cells correlates with the kindling model for epilepsy. Calbindin D-28K immunoreactivity is shown to be specifically absent from neuronal populations lost early in ALS, while substantial loss of neurons, which do contain this protein occur in patients with Huntington′s disease. (Ref.: Schmidt, H (2012). Front. Mol. Neurosci. 5; 25).

Immunogen

Epitope: C-terminus

GST/His-tagged recombinant fragment corresponding to 107 amino acids from the C-terminal end of murine Calbindin D-28K.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Format: Purified

Quality

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Calbindin D-28K in human brain tissue lysate.

Specificity

This rabbit polyclonal antibody detects Calbindin D-28K in Human, Murine, and Rat cells. It targets an epitope within 107 amino acids from the C-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~28 kDa observed; 29.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).