Application

This IL-12 alpha antibody is validated for use in WB & IHC for the detection of the IL-12 alpha protein.

Immunohistochemistry Analysis: A 1:16,000-32,000 dilution from a representative lot detected IL-12 alpha in human tonsil and human brain tissue.

General description

Interleukin-12 alpha (IL-12A) is also known as Cytotoxic lymphocyte maturation factor 35 kDa subunit (CLMF p35), IL-12 subunit p35, or NK cell stimulatory factor chain 1 (NKSF-1). IL-12A is a cytokine that, along with IL12B, forms a disulfide-linked heterodimer interleukin IL-12. IL-12 alpha functions as a regulator of T cell mediated cytotoxicity, interferon-gamma production, lymphocyte proliferation and activation of natural killer cells.

Immunogen

KLH-conjugated linear peptide corresponding to the near C-terminus of human IL-12 alpha.

Linkage

Replaces: MABN1084

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blotting in human lymph node tissue lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected IL-12 alpha in 10 µg of human lymph node tissue lysate.

Specificity

This antibody recognizes the near C-terminus of IL-12 alpha

Target description

~25 kDa observed