Analysis Note

ControlARPE19 tissue lysate

Application

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected TTC8 in human prostate cancer and mouse eye tissues.

Research Sub CategoryCytoskeleton

Research CategoryNeuroscience

Anti-TTC8 detects levels of TTC8 proteins & has been published & validated for use in WB & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Also known as tetratricopeptide repeat protein 8, TTC8 is a BBSome complex that is required for ciliogenesis. It is known to associate with the Rab8 GDP/GTP exchange factor, which is localized in the basal body. Rab8(GTP) is produced and enters the primary cilium, where it promotes ciliary membrane biogenesis. Mutations in TTC8 have been identified in Bardet-Biedl syndrome (BBS), a genetic disorder which is characterized with obesity, retinal degeneration, and nephropathy.

Immunogen

KLH-conjugated linear peptide corresponding to the TPR1 domain of human TTC8.

Epitope: TPR1 domain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in ARPE19 tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected TTC8 in 10 µg of ARPE19 tissue lysate.

Specificity

This antibody recognizes the TPR1 domain of TTC8.

Other homologies: Rat (53% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~61 kDa observed. Uncharacterized bands at ~270 kDa, ~160 kDa, and ~38 kDa may be observed in some cell lysates.