Analysis Note

ControlPositive Control: Heart tissue, mouse brain tissue lysate.

Application

Immunoprecipitation:
2-10 µg of a previous lot was used in immunoprecipitation.

ELISA:
A previous lot of this antibody was used at 1:10,000-100,000 dilution using 50 - 100 ng Cx43 control peptide (Catalog number AG636) per well.

Optimal working dilutions must be determined by end user.

Anti-Connexin 43 Antibody, C-terminus, cytosolic is an antibody against Connexin 43 for use in ELISA, IF, IH, IP & WB.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mouse Connexin 43 is a 382 amino acid gap junction protein with a predicted M.W. of ~43 kDa. It is prominently expressed in heart (see reviews: Kumar & Giula 1996; White et al. 1995; Evans 1994; Beyer et al. 1990).

Connexin 43 (GJA1) is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. GJA1 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. GJA1 is targeted by several protein kinases that regulate myocardial cell cell coupling. A related intronless GJA1 pseudogene, GJA1P, has been mapped to chromosome 5. Mouse Connexin 43 is a 382 amino acid gap junction protein with a predicted M.W. of ~43 kDa. It is prominently expressed in heart (Kumar, N. and Giula, N., 1996, Cell 84: 381-388).

Immunogen

Epitope: C-term/cytosolic

Anti-Connexin 43 is made against a 23 amino acid C-terminal peptide sequence within the cytoplasmic domain of mouse C×43 (Beyer, E. and Steinberg, T. ,1991, JBC 266: 7971).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

ImmunoAffinity Purified

Purified rabbit polyclonal in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl, with 0.1% sodium azide as a preservative.

Quality

Routinely evaluated by Western Blot on Huvec lysates.

Western Blot Analysis: 1:500 dilution of this lot detected CONNEXIN 43 on 10 µg of Huvec lysates.

Specificity

Mouse C×43 immunogenic peptide sequence is specific for C×43 and no significant homology is seen with other connexins. The mouse C×43 peptide sequence is 100% homologous with rat and human C×43 (Beyer, E. et al., 1985, JCB 105: 2621).

Recognizes mouse Connexin 43.

Storage and Stability

Stable for up to 1 year at 2-8°C in undiluted aliquots from date of receipt.

Target description

43 kDa