Application

This Anti-Connexin 43 Antibody is validated for use in ELISA, IP, WB, IC for the detection of Connexin 43.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

ELISA: 1:10,000-100,000 using 50 - 100 ng Cx43 control peptide per well. Immunocytochemistry: not tested. It is recommended that the antibody be tried at 2-20µg/mL in formaldehyde fixed (Beyer et al. 1985; Nicholson et al. 1985; John et al. 1991; Fishman et al. 1990). Immunoblotting: 1-10µg/mL using Chemiluminescence technique. Optimal working dilutions must be determined by end user.Immunohistochemistry: A 1:50 dilution of this antibody detected Connexin 43 in sections from mouse heart tissue pretreated with Tris-EDTA buffer, pH 9.0. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mouse Connexin 43 is a 382 amino acid gap junction protein withmouse connexin

a predicted M.W. of ~43 kDa. It is prominently expressed in heart (see reviews: Kumar & Giula 1996; White et al. 1995; Evans 1994; Beyer et al. 1990).

Immunogen

KLH-conjugated synthetic peptide
corresponding to amino acids 360-382 within
the C-terminus of mouse connexin 43.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Affinity-purified using peptide-Sepharose column chromatography and supplied in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl containing 0.05% sodium azide.

Specificity

Mouse Cx43 immunogenic peptide sequence is specific for Cx43 and no significant homology is seen with other connexins. The mouse Cx43 peptide sequence shows 100% conserved with rat and bovine, and 84% with chicken and human (16/19 aa) Cx43 (Beyer et al. 1985; Nicholson et al. 1985; John et al. 1991; Fishman et al. 1990).

Storage and Stability

Store at 2-8°C for 1 year from date of receipt.