Western blot: 1:1000-1:2000
Human K562 cell lysate can be used as a positive control and a 23 kDa band can be detected.
Optimal working dilutions must be determined by end user.
Research CategoryApoptosis & Cancer
Research Sub CategoryBCL2 & Inhibition
This Anti-Bim Antibody, internal epitope, pan-Bim isoforms is validated for use in WB for the detection of Bim.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Epitope: internal epitope, pan-Bim isoforms
Synthetic peptide corresponding to amino acids 22-40 of human origin (O′Conneor et al. 1998). The sequence is identical to that of mouse and is different by one amino acid from that of rat.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Immunoaffinity Purified immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Recognizes pan-Bim isoforms.
Storage and Stability
The undiluted antibody solution is stable for approximately 6 months when stored 2–8°C.