A9316-.25ML Display Image

Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody produced in goat, affinity isolated antibody, buffered aqueous glycerol solution

Code: A9316-.25ML D2-231

Application

Surfactant Protein A was detected in bronchoalveolar fluid using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary at µg/ml in TBS/Tween contai...

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Application

Surfactant Protein A was detected in bronchoalveolar fluid using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary at µg/ml in TBS/Tween containing final concentration of 0.5M NaCl.

The level of antibodies specific to P. aeuginosa present in immunized mouse sera was determined by ELISA using alkaline phosphatase-conjugated goat anti-mouse IgG as the secondary antibody at a dilution of 1:1000.

Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in goat has been used as a secondary antibody in:• histological studies in heart and liver grafts• immunohistochemical studies of gangliosides and immunostaining of lymphoid organs• western blot detection in blood samples

Biochem/physiol Actions

IgG1 deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant immunoglobulin present in human serum. The four classes of IgG are IgG1, IgG2, IgG3, and IgG4.

Goat polyclonal anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody is reactive with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM. Cross reactivity of the antibody conjugate is determined by ELISA. The conjugate shows no reactivity with rat IgG or rat serum proteins.

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.

Other Notes

Antibody adsorbed with rat serum proteins.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 10 mM glycine, 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background with rat samples.

Quality Level200
storage temp.2-8°C
clonepolyclonal
shipped inwet ice
antibody formaffinity isolated antibody
application(s)direct ELISA: 1:30,000
application(s)western blot: 1:30,000
application(s)immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
conjugatealkaline phosphatase conjugate
antibody product typesecondary antibodies
biological sourcegoat
formbuffered aqueous glycerol solution

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