Application

Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.

Egg white avidin has been used as a cross-linker for biotin-conjugated antibodies. The product has also been used as a blocking agent in ELISA applications.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Packaging

1, 2, 5 mg in glass bottle

10, 25, 100 mg in poly bottle

Preparation Note

Affinity purified

Unit Definition

One unit will bind 1.0 µg of d-biotin.