Application

This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid whencoupled with the related enzymes in clinical analysis.For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.

Used in the Sialic Acid Quantification Kit, SIALIC-Q

Packaging

25 units in glass bottle

Physical form

Lyophilized powder containing mannitol and EDTA

Physical properties

Isoelectric point: 4.6 ± 0.1Michaelis constant: 2.5 x 10‾³M (N-Acetylneuraminic acid)Structure: 3 subunits (approx. 35,000) per mol of enzymeInhibitors: p-Chloromercuribenzoate, sodium dodecyl sulfact, Hg⁺⁺, Ag⁺ Optimum pH: 7.5– 8.0Optimum temp: 70°CpH Stability: pH 6.0–9.0 (10°C, 25hr)Thermal stability: Below 65°C (pH 7.5, 30 min)

Unit Definition

One unit will release 1.0 µmole of pyruvate from NANA per min at pH 7.7 at 37 °C.