Application

4-Amidinophenylmethanesulfonyl fluoride hydrochloride has been used:as a component of inhibitor cocktail for whole blood collection and separation of plasmaas a component of sodium dodecyl sulfate (SDS) sample buffer to prepare whole-cell extractas a trypsin inhibitor to measure its inhibition efficiency and to determine the efficacy of the quantum dot fluorescence resonance energy transfer (FRET)-based enzymatic probes

Biochem/physiol Actions

4-Amidinophenylmethanesulfonyl fluoride hydrochloride (p-APMSF) can inhibit the trypsin-like proteinase produced by Fusarium culmorum. It is also capable of blocking bovine Factor Xa, human plasmin, and the human complement proteases, C1r and C1s.

Irreversible inhibitor of serine proteases with lysine or arginine substrate specificities. Effective concentration 10-100 µM. Frequently used to characterize newly discovered proteases.

General description

4-Amidinophenylmethanesulfonyl fluoride hydrochloride is relatively nontoxic.

Packaging

10 mg in glass bottle

50 mg in poly bottle