Detection of TLR2 and TLR4 in protein extracts from epidermal keratinocytes was performed by western blot using alkaline phosphatase conjugated rabbit anti-goat IgG as the secondary at a 1:2500 dilution.
Anti-Goat IgG (whole molecule)-Alkaline Phosphatase antibody is suitable for use in immunoblotting. The product can also be used for direct ELISA (1:30,000) and immunohistochemistry (1:50 using formalin-fixed, paraffin-embedded sections).
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IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus they play a crucial role in facilitating cytological immune responses. Polyclonal anti-goat IgG (whole molecule)–alkaline phosphatase antibody (diluted 1:15,000) can be used as a secondary antibody for immunoblotting of Sall4. This antibody can also be used in immunohistochemistry (diluted1:400) . Rabbit anti-goat IgG antibody reacts specifically with goat IgG and normal goat serum.
Primary goat antibodies are often used to study target proteins for various clinical and research purposes. Thus, secondary anti-goat antibody conjugated to a detectable substrate can be used to facilitate the accurate detection and localization of target proteins.
Purified goat IgG.
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.
Binds all goat Igs