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Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody produced in goat, affinity isolated antibody, buffered aqueous glycerol solution

Code: A3562-1ML D2-231

Application

Surfactant Protein A was detected in bronchoalveolar fluid using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary at µg/ml in TBS/Tween contai...

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£308.00 1ML
List Price

Application

Surfactant Protein A was detected in bronchoalveolar fluid using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary at µg/ml in TBS/Tween containing final concentration of 0.5M NaCl.

Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody is suitable for use in direct ELISA (1:3000) and western blot. The product can also be used for immunohistochemistry (1:50 using formalin-fixed, paraffin-embedded sections).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody is specific for normal mouse serum and mouse IgG. In Ouchterlony double diffusion assays, the antibody reacts with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM.

Immunogen

Purified mouse IgG

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.

species reactivitymouse
Quality Level200
storage temp.2-8°C
clonepolyclonal
shipped inwet ice
antibody formaffinity isolated antibody
application(s)direct ELISA: 1:30,000
application(s)dot blot: 1:30,000
application(s)western blot: 1:30,000
application(s)immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
conjugatealkaline phosphatase conjugate
antibody product typesecondary antibodies
biological sourcegoat
formbuffered aqueous glycerol solution