81189-1KG Display Image

Poly(ethylene glycol), BioUltra, for molecular biology, 1,000

Code: 81189-1KG D2-231

Application

Poly(ethylene glycol) has been used to develop a method for efficient transformation of Brevibacterium lactofermentum protoplasts with plasmid DNA. It ha...


read more

Your Price
£209.00 1KG
REQUEST

Application

Poly(ethylene glycol) has been used to develop a method for efficient transformation of Brevibacterium lactofermentum protoplasts with plasmid DNA. It has been used as a fusion agent to fuse spleen cells with the myeloma cell line SP2/0.

General description

Poly(ethylene glycol) (PEG) is a non-ionic hydrophilic polymer and is available in different molecular weights. It helps in the purification and crystal growth of proteins and nucleic acids. PEG and dextran together result in aqueous polymer two phase system, which is required for the purification of biological materials. PEG also interacts with cell membrane, thereby allowing cell fusion.

Other Notes

PEG precipitation of DNA fragments

λ50 mg/mL in H2O
anion tracessulfate (SO42-): ≤50 mg/kg, chloride (Cl-): ≤50 mg/kg
cation tracesBi: ≤5 mg/kg, Mo: ≤5 mg/kg, Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Li: ≤5 mg/kg, K: ≤200 mg/kg, Na: ≤200 mg/kg, Cr: ≤5 mg/kg, Mn: ≤5 mg/kg, Sr: ≤5 mg/kg, Mg: ≤5 mg/kg, Ca: ≤10 mg/kg, Pb: ≤5 mg/kg, Cu: ≤5 mg/kg, Ba: ≤5 mg/kg, Fe: ≤5 mg/kg, Zn: ≤5 mg/kg, Ni: ≤5 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg
formsolid
gradefor molecular biology
impuritiesphosphatases, none detected, RNases, none detected, proteases, none detected, insoluble matter, passes filter test, DNases, none detected
InChI keyLYCAIKOWRPUZTN-UHFFFAOYSA-N
InChI1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
mol wtMr 950-1050
pH5.5-7.0 (25 °C, 50 mg/mL in H2O)
product lineBioUltra
Quality Level200, 100
SMILES stringC(CO)O
solubilityH2O: 50 mg/mL at 20 °C, clear, colorless
UV absorptionλ: 260 nm Amax: ≤0.10, λ: 280 nm Amax: ≤0.05
Code
Description
Unit Size
List Price
Qty
81189-100G
Poly(ethylene glycol), BioUltra, for molecular biology, 1,000

Unit:100G
List Price: £43.10
Source:List Price
ADD
81189-250G
Poly(ethylene glycol), BioUltra, for molecular biology, 1,000

Unit:250G
List Price: £64.30
Source:List Price
ADD
Cas Number25322-68-3
This product has met the following criteria: