Application

16:0-10 Doxyl PC may be used:in vesicles for quenching PT-(1–46)F4W fluorescenceas vesicle component for tryptophan based fluorescence emission studiesto probe the oxygen and Ni[II] ethylenediaminediacetic acid (NiEdda) accessibilities inside liposomes

Biochem/physiol Actions

Phosphatidylcholine (PC) functions as a surfactant within the mucus to form a hydrophobic surface to inhibit bacterial penetrance.

General description

Avanti′s nitroxide spin product listing is a group of compounds designed to act as membrane probes. A variety of positions down the hydrophobic chain are labeled with the nitroxide functional groups to allow probing the membrane at various depths. These compounds have been synthesized from 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphocholine with the product being purified by column chromatography. Various n-doxyl phosphocholines have been recently used as biophysical tools to elucidate membrane trafficking with phosphatidylinositol transfer proteins and as fluorescent quenchers in lipid bilayer structural studies.

Phosphocholine is considered as a precursor molecule. It is formed during the breakdown of phosphatidylcholine metabolism. 10-Doxyl is a spin label probe.

Packaging

5 mL Clear Glass Sealed Ampule (810603P-1mg)

Preparation Note

Product use: To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies. For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 μl absolute ethanol for a concentration of 40.3 mM , Additional supplemental information.