Cell Line Description
DerivationPrimary cell type: -Reprogramming methodVector type: Non-integratingVector: EpisomalGene list:KLF4Lin28MYCPOU5F1SOX2Is the reprogramming vector detectable: NoMerthods used: pcrXeno free conditions: noDerived under gmp: noAvailable as clinical grade: noCharacterizationAnalysis of Undifferentiated CellsMorphology:03-08-2017 16423 apoe 22.tifMicrobiology / Virus ScreeningHIV 1: NegativeHIV 2: NegativeHepatitis B: NegativeHepatitis C: NegativeMycoplasma: NegativeGenotypingKaryotypingPassage number: 23Cell line karyotype: 46 XYKaryotyping method: G-BandingImage / ResultGenetic ModificationDisease/phenotype related modificationsDisease: Alzheimer’;s diseaseType of modification: IsogenicChromosome location: 19q13.32Nucleotide sequence HGSV: rs7412 (T/T); rs429358 (T/T)Protein sequence HGSV: 112Cys, 158CysZygosity: HomozygousTarget locus modification: The bases at both SNPs rs7412 and rs429358 were changed to a T
Cell Line Origin
DepositorBioneer
General description
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer’;s Disease, Parkinson’;s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington’;s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
Legal Information
EBiSC is a trademark of Fraunhofer-Gesellschaft
E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
Linkage
Note: EAUA and CLIP must be completed before order fulfillment
Additional Cell Line Info EBiSC Access Use Agreement (EAUA) Cell Line Information Pack (CLIP)
Subculture Routine
Surface coating: Matrigel®/Geltrex®Culture medium: Essential E8®
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