Cell Line Description

DerivationPrimary cell type: Peripheral blood mononuclear cellTissue collection year: 2016Reprogramming methodVector type: Non-integratingVector: Sendai virusGene list:KLF4MYCPOU5F1SOX2Is the reprogramming vector detectable: NoMerthods used: pcrFiles showing reprogramming vector expressed or silenced: Image / ResultVector map: FileVector Free ReprogrammingTypes of factor(s) used: mRNAXeno free conditions: noDerived under gmp: noAvailable as clinical grade: noCharacterizationAnalysis of Undifferentiated CellsMarker expression:Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression ProfilesTRA 1-60 Yes -TRA 1-81 Yes -hPSC ScorecardPositive NegativeSelf-renewalEndodermMesodermEctodermData analysis report: scorecard_mv.pngMorphology:Microbiology / Virus ScreeningHIV 1: NegativeHIV 2: NegativeHepatitis B: NegativeHepatitis C: NegativeMycoplasma: NegativeGenotypingKaryotypingPassage number: 31Cell line karyotype: normalKaryotyping method: Molecular karyotyping by SNP arrayImage / Result

Cell Line Origin

DepositorUniversita¨tsklinikum Bonn

General description

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer’;s Disease, Parkinson’;s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington’;s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Legal Information

StemMACS is a trademark of Miltenyi Biotec GmbH

EBiSC is a trademark of Fraunhofer-Gesellschaft

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.

Linkage

Additional Cell Line Info EBiSC Access Use Agreement (EAUA) Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

Subculture Routine

Surface coating: Matrigel^®/Geltrex^®Passage method: Enzyme-free cell dissociation / EDTAO₂ concentration: 21%CO₂ concentration: 6%Culture medium: otherCulture base medium: StemMACS^™ iPS-Brew XFMain protein source: -Passage Number banked (pre-EBiSC): 31No. Vials banked (pre-EBiSC): 30