Cell Line Description

DerivationPrimary cell type: Adipose tissue derived mesenchymal stem cellPassage number reprogrammed: 3Reprogramming methodVector type: Non-integratingVector: EpisomalGene list: KLF4LIN28MYCPOU5F1shP53SOX2Is the reprogramming vector detectable: NoMerthods used: pcrVector map: FileCharacterizationAnalysis of Undifferentiated CellsMarker expression: SSEA-4(+)SOX2(+)TRA 1-81(+)SSEA-1(-)TDG1(+)POU5F1 (OCT-4)(+)NANOG(+)DMNT3B(+)GDF3(+)GABRB3(+)Differentiation potencyEctoderm: In vitro directed differentiationMarker Expressed:Nestin(+)Pax6(+)Endoderm: In vitro directed differentiationMarker Expressed:CD184(+)Sox17 (+)Mesoderm: In vitro directed differentiationMarker Expressed:CD34 (+)CD56(+)Microbiology / Virus ScreeningHIV 1: NegativeHIV 2: NegativeHepatitis B: NegativeHepatitis C: NegativeMycoplasma: NegativeSterilityInoculation for microbiological growth: No Contaminants DetectedMycoplasma: Not DetectedViability: Viable post-cryopreservationGenotypingKaryotypingPassage number: 12Cell line karyotype: 46, XY, normal male karyotypeKaryotyping method: G-BandingGenotypingSTR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Cell Line Origin

DepositorBioneer

General description

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer’;s Disease, Parkinson’;s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington’;s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Legal Information

EBiSC is a trademark of Fraunhofer-Gesellschaft

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.

Linkage

Note: EAUA and CLIP must be completed before order fulfillment

Additional Cell Line Info EBiSC Access Use Agreement (EAUA) Cell Line Information Pack (CLIP)

Subculture Routine

Medium: Essential E8^®Passage method: EDTAMatrix: Matrigel^® / Geltrex^®CO₂ concentration: 5%O₂ concentration: 5%Temperature: 37°C