Features and Benefits

X-tremeGENE HP DNA Transfection Reagent is a high performance reagent that transfects a broad range of eukaryotic cells, including insect cells and hard-to-transfect cell lines (e.g., Sf9, U-2 OS, MEF, MCF7, Hep G2, PC-3, HeLa, HT-29, HT-1080, K-562, RAW 264.7, Jurkat, PC-12, and HCT 116). Benefit from an easy-to-use non-liposomal reagent that is free of animal-derived components, stable at room temperature, filtered through 0.2 µm pore size membrane, and active in serum-containing medium. Achieve new levels of transfection efficiency in primary cells and tumor cell lines that are not transfected well by other reagents. Generate physiologically relevant data using a reagent with low cytotoxic effects. Increase experimental throughput and enable target evaluation using a simple and consistent protocol.

General description

X-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.

Legal Information

X-tremeGENE is a trademark of Roche

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 µm pore size membrane. Does not contain any ingredients of human or animal origin.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE HP DNA Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

Quality

Each lot of X-tremeGENE HP DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE HP DNA Transfection Reagent (ratio 3:1 ¼l/¼g DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.