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Magnesium chloride solution, BioUltra, for molecular biology, approx. 1 M in H2O

Code: 63069-500ML D2-231

Application

Magnesium chloride solution has been used as a component of nuclei isolation buffer for in situ fixations and isolation of nuclei from Kupffer cells (or ...


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Application

Magnesium chloride solution has been used as a component of nuclei isolation buffer for in situ fixations and isolation of nuclei from Kupffer cells (or other liver cells).

Magnesium chloride solution has been used in the reaction mix of reverse transcriptase enzyme, PFV RT. It has been used in the preparation of artificial cerebrospinal fluid (ACSF).

General description

Magnesium chloride is widely used in polymerase chain reactions (PCR) buffers to supply magnesium ions. The concentration of Mg2+ is the most critical parameter that impacts both the efficiency and specificity of PCR when total genomic DNA is used, synthesis of long products, or during multiplex reactions. Elevated concentrations of Mg2+ enhance the stability of primer-template duplexes and the efficiency of amplification.

λ1 M in H2O
anion tracessulfate (SO42-): ≤50 mg/kg, phosphate (PO43-): ≤10 mg/kg
cation tracesCd: ≤1 mg/kg, Ca: ≤10 mg/kg, Fe: ≤1 mg/kg, Pb: ≤1 mg/kg, K: ≤50 mg/kg, Bi: ≤1 mg/kg, As: ≤0.1 mg/kg, Co: ≤1 mg/kg, Ba: ≤1 mg/kg, Cu: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Sr: ≤1 mg/kg, Al: ≤1 mg/kg, Na: ≤500 mg/kg, Ni: ≤1 mg/kg, Li: ≤1 mg/kg, Cr: ≤1 mg/kg, Zn: ≤1 mg/kg
concentration~1 M in H2O
density1.08 g/mL at 20 °C
gradefor molecular biology
impuritiesinsoluble matter, passes filter test, ≤0.0002% total Nitrogen (N), phosphatases, none detected, RNases, none detected, DNases, none detected, proteases, none detected
InChI keyTWRXJAOTZQYOKJ-UHFFFAOYSA-L
InChI1S/2ClH.Mg/h2*1H;/q;;+2/p-2
pH5.0-7.5 (25 °C, 1 M in H2O)
product lineBioUltra
Quality Level200
SMILES stringCl[Mg]Cl
UV absorptionλ: 280 nm Amax: 0.03, λ: 260 nm Amax: 0.05
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63069-100ML
Magnesium chloride solution, BioUltra, for molecular biology, approx. 1 M in H2O

Unit:100ML
List Price: £98.00
Source:List Price
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Cas Number7786-30-3
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