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SUPELCOSIL LC-NH2 HPLC Column, 5 mum particle size, L x I.D. 25 cm x 4.6 mm

Code: 58338 D2-231

General description

The amino column is most often employed for the separation of mono- and disaccharides. As a normal-phase application, amino columns are used in the petrol...


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General description

The amino column is most often employed for the separation of mono- and disaccharides. As a normal-phase application, amino columns are used in the petroleum industry (see SUPELCOSIL™ LC-NH2-NP HPLC Columns for additional details).

Silica-based SUPELCOSIL LC-NH2 columns separate monosaccharides, disaccharides, and some trisaccharides, with elution generally in order of increasing molecular weight. Retention decreases as the proportion of water:acetonitrile in the mobile phase is increased. For more information, request T397126 (Sugars on SUPELCOSIL LC-NH2 Columns).

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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agencysuitable for USP L8
application(s)food and beverages
extent of labeling3% Carbon loading
featureendcapped
L × I.D.25 cm × 4.6 mm
manufacturer/tradenameSUPELCOSIL™
matrix active groupamino phase
matrixsilica gel, spherical particle platform
parameter≤70 °C temp. range, 400 bar pressure (5801 psi)
particle size5 µm
pore size120 Å
Quality Level100
separation techniquenormal phase, hydrophilic interaction (HILIC)
surface area170 m2/g
surface coveragesurface coverage 5.1 µmol/m2
technique(s)HPLC: suitable
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