Application

ELISA (0.05 µg/ml)r>Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)r>Immunocytochemistry (1 µg/ml, immunofluorescence)

General description

Purified mouse monoclonal antibody. Recognizes the ~60 kDa Src protein phosphorylated at Tyr⁴¹⁸ (human, rat), Tyr⁴²³ (mouse), and Tyr⁴¹⁶ (chicken, v-Src).

Recognizes the ~60 kDa Src protein phosphorylated at Tyr⁴¹⁸ in chick embryo fibroblasts expressing human wild-type Src.

This PhosphoDetect™ Anti-Src (pTyr⁴¹⁸) Mouse mAb (9A6) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of Src (pTyr⁴¹⁸).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the pTyr⁴¹⁸ phosphorylation site of human Src

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Martin, G.S. 2001. Nat. Rev. Mol. Cell Biol.2, 467.Reissig, D., et al. 2001. J. Cancer Res. Clin. Oncol.127, 226.Bjorge, J.D., et al. 2000. Oncogene19, 5620.Irby, R.B. and Yeatman, T.J. 2001. Oncogene19, 5636.Miermont, A.M., et al. 2000. J. Immunol. Methods246, 203.

Does not cross-react with non-phosphorylated Src, or with unrelated phosphorylation sites. The sequence of the immunogen is conserved in human (Tyr⁴¹⁸), mouse (Tyr⁴²³), rat (Tyr⁴¹⁸), chicken (Tyr⁴¹⁶), and c-Src (Tyr⁴¹⁶). Variables associated with assay conditions will dictate the optimal working dilution.

Packaging

100 µg in Plastic ampoule

Physical form

Lyophilized from 2X PBS, PEG, sucrose.

Reconstitution

Reconstitute in 1 ml sterile dH₂O (15 min, room temperature). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.

Warning

Toxicity: Standard Handling (A)