Application

ELISA (see comments)r>Immunoblotting (1:1000)r>Immunocytochemistry (see comments)

General description

This PhosphoDetect™ Anti-Insulin Receptor (pTyr^1162/1163) Rabbit pAb is validated for use in ELISA, WB, ICC for the detection of Insulin Receptor (pTyr^1162/1163).

Note: 1 T = 1 test. Sufficient for 10 Western mini blots.

Rabbit polyclonal antibody adsorbed with non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the insulin receptor (IR) protein phosphorylated at Tyr^1162/1163 and insulin-like growth factor 1 receptor (IGF-1R) protein phosphorylated at Tyr^1162/1163.

Recognizes the ~95 kDa β subunit of the insulin receptor protein phosphorylated at Tyr¹¹⁶² and Tyr¹¹⁶³ in insulin receptor transfected cells. Cross-reacts with the insulin-like growth factor-1 receptor phosphorylated at Tyr¹¹³⁵ and Tyr¹¹³⁶.

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr^1162/1163 phosphorylation sites of human insulin receptor

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

The immunogen sequence is 100% conserved in the mouse and rat insulin recptor protein, but cross-reactivity has not been tested. This antibody has also been reported to work for ELISA and immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.

Bevan, P. 2001. J. Cell Sci.114, 1429.Ottensmeyer, F.P., et al. 2000. Biochemistry39, 12103.Virkamaki, A., et al. 1999. J. Clin. Invest.103, 931.Schmid, E., at al. 1998. FASEB J.12, 863.Hari, J. and Roth, R.A. 1987. J. Biol. Chem.262, 15341.Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA83, 328.White, M.F. and Kahn, C.R. 1986. The Enzymes17, 247.Ebina, Y., et al. 1985. Cell40, 747.Ganguly, S., et al. 1985. Current Topics in Cellular Regulation27, 83.Grunberger, G., et al. 1984. Science223, 932.

Packaging

10 tests in Plastic ampoule

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)