Application

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is an amine reactive fluorescent probe used to stain cells, especially immune cells, for analysis in applications such as flow cytometry. Forms green fluorescent conjugates on deacetylation.

Amine-reactive label for long term tracing of cells. Forms green fluorescent conjugates on deacetylation.

5-Carboxyfluorescein diacetate N-succinimidyl ester has been used:to label cells for flow cytometryin lymphocyte proliferation assayto determine the intracellular or intra-organellar pH in chloroplaststo label Leishmania amazonensis and Leishmania major

Biochem/physiol Actions

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.

General description

5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.