Biotin has been used for testing the functionality of the Avidin-displaying phages. The product has also been used for labeling the swine anti-rabbit IgG antibody and peroxidase.
Biotin also referred to as vitamin H or vitamin B7 is a water-soluble B-vitamin that induces two to three fold increase in guanylate cyclase activity in rat liver, kidney, colon, cerebellum, and heart. It also stimulates the sperm motility and prolongs the survival of cryopreserved human spermatozoa.
Biotin acts as an important cofactor for carboxylase enzymes in various metabolic pathways. Biotin favors keratin synthesis and hence is used as a supplement for hair and nail growth. Deficiency of biotin shows several symptoms such as alopecia, conjunctivitis, seborrheic dermatitis, depression, lethargy, hypotonia and seizures.
Biotin is readily available in several foods, like whole grains, nuts, legumes, unpolished rice and egg yolk. The normal gut flora also produces this vitamin.
Reviews; Avidin-Biotin technology;