Application

Use BCIP in alkaline phosphatase detection: Southern blotNorthern blotWestern blotColony and plaque hybridizationIn situ hybridization

General description

BCIP serves as a substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Solution (50 mg/ml) in dimethylformamide (DMF)

Principle

Use of BCIP as substrate for alkaline phosphatase in combination with nitro blue tetrazolium chloride (NBT) as electron acceptor: Substrates and reaction products of alkaline phosphatase catalyzed color reaction with NBT/BCIP.

Quality

Function test: incubation with AP