Application

Astec^® CHIROBIOTIC^® V Chiral HPLC column may be used for the separation of different types of racemic compounds (ß-blockers, ß-agonists, benzodiazipines, non-steroidal anti-inflammatory drugs, barbiturates, free and derivatized amino acids in packed column subcritical fluid chromatography (pSFC). It may also be used for the separation of citalopram enantiomers and its two N-demethylated metabolites and of alprenolol that is the internal standard, in human plasma using chiral liquid chromatography. It may also be used in the chiral liquid chromatography analysis of ketoprofen and flurbiprofen.

General description

Neutral molecules, amides, acids, esters and amines show considerable enantioselectivity on these vancomycin-based CSPs. A wide variety of secondary and tertiary amines have been separated on the CHIROBIOTIC^® V in the polar ionic mode. The CHIROBIOTIC^® V has demonstrated many of the separation characteristics of protein-based stationary phases, but with exceptional stability and much higher sample capacity. Some chiral analytes have been resolved that have not been reported separated on any other chiral stationary phase. CHIROBIOTIC^® V and V2 differ in their bonding chemistry the pore size of the support particle, giving them different selectivity and preparative capacity. Bonded phase: Vancomycin Operating pH range: 3.5 - 7.0 Particle diameter: 5, 10 or 16 µm Pore size: 100 Å (CHIROBIOTIC^® V) or 200 Å (CHIROBIOTIC^® V2)

Legal Information

CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Astec is a registered trademark of Sigma-Aldrich Co. LLC

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