Analysis Note

Absorbance coefficients: 18.0 x 10³ [l x mol^-1 x cm^-1] at 260 nm, 5.9 x 10³ [l x mol^-1 x cm^-1] at 333 nm (CN complex)Absorbance at 340 nm: Imidazol buffer, 100 mM pH 6.7, NADP 2 mM (= 14.87 mg/10 ml) : A₃₄₀ approximately 0.100.Absorbance at 360 nm: Aqueous solution of NADP, 10 mg/ml: A₃₆₀ approximately 0.200-0.250.

Application

The β-NADH phosphate disodium salt has been used for the quantification of glucose in glycogen. It has also been used for the hexokinase assay.

General description

Nicotinamide adenine dinucleotide phosphate (NADP) and NADPH form a redox pair. The NADPH/NADP ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Storage conditions (working solution): 2 to 8 °C10 mg/ml in aqueous solution, contains at least 99% NADP after 4 weeks at 2 to 8 °C