Application

Endothelial vessel function, cell signaling, angiogenesis

Cell Line Origin

Skin

Components

Cell Basal Medium containing 10% FBS & 10% DMSO

General description

CADMEC/HMVEC provide an excellent model system to study many aspects of endothelial function and disease, especially those related to the microvasculature and capillary systems. HMVEC/CADMEC have been shown to express vWF, CD36 and CD31, are able to uptake DiI-Ac-LDL and are positive for functional activity by cytokine-stimulated leukocyte adherence.Select HMVEC lots have been additionally tested to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx).

Preparation Note

2nd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO Can be cultured at least 16 doublings

Subculture Routine

Please refer to the CADMEC/HMVEC Culture Protocol.