P9582-5MG Display Image

Polyinosinic-polycytidylic acid potassium salt, with buffer salts, TLR ligand tested

Code: P9582-5MG D2-231

Application

TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. Polyinosinic–polycytidylic acid (Poly(I) • Poly(C)) is...

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£72.60 5MG
List Price

Application

TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. Polyinosinic–polycytidylic acid (Poly(I) • Poly(C)) is a double-stranded homopolymer used as a model RNA to study cell signaling at the level of TLR3. Poly(IC) is a TRIF-dependent toll-like receptor-3 (TLR3) ligand.

Biochem/physiol Actions

Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine.

Other Notes

Double-stranded homopolymer.

Packaging

5, 50 mg in glass bottle

Package size based on polynucleotide content

Preparation Note

The product requires ionic strength to maintain the double-strand structure. Reconstitution at ~10 mg/mL of water yields a polynucleotide in physiological phosphate buffered solution.

Quality Level300
assay≥99% (less than 1% free nucleotides, TLC)
compositionPoly(I) Poly(C), 10% (balance buffer salts as sodium chloride and sodium phosphate)
storage conditiondesiccated
formlyophilized powder
storage temp.−20°C

Code
Description
Unit Size
List Price
Qty
P9582-50MG
Unit:50MG
List Price: £412.00
Source:List Price
ADD
Cas Number31852-29-6