General description

Full length, recombinant, human Plk2 fused at the N-terminus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. Plk2 is a G1 specific kinase that is specifically detected in brain, heart, and lung. Plk2 expression increases in the presence of DNA-damaging agents. The active kinase is useful for the study of Plk2 regulation and inhibitor screening.

Legal Information

TWEEN is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Eckerdt, F., et al. 2005. Oncogene24, 267.Warnke, S., et al. 2004.Curr Biol.14, 1200.

Packaging

10 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% Tween^®-20 detergent, pH 8.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will phosphorylate 1 pmol SER/THR 16 substrate per min at 30°C, pH 7.5.

Warning

Toxicity: Standard Handling (A)