Protein determined by Warburg-Christian
Phosphodiesterase, 3',5'-cyclic-nucleotide-specific from bovine brain has been used to assay the protein activator, calmodulin and in in vitro adenylate cyclase assay.
Hydrolyzes the 3′,5′-phosphodiester in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.
Phosphodiesterase (PDE1) exists in two isoforms -PDE1A2 and PDE1B1. The enzyme has a role in the complex interaction between cyclic nucleotides and Ca2+ second-messenger systems.
Package size based on activated units
Lyophilized powder containing Tris-HCl buffer salts and lactose
Affinity purified preparation which has been depleted of calmodulin.
Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −20 °C. However, the calmodulin-deficient activity may increase up to 200%. Both the activated and calmodulin-deficient activity may decrease approximately 30% in 24 hours if stored at 4 °C.
One unit will hydrolyze 1.0 µmole of 3′,5′-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.