ELISA (0.05 µg/ml)r>Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)r>Immunocytochemistry (1 µg/ml, immunofluorescence)
Purified mouse monoclonal antibody. Recognizes the ~60 kDa Src protein phosphorylated at Tyr418 (human, rat), Tyr423 (mouse), and Tyr416 (chicken, v-Src).
Recognizes the ~60 kDa Src protein phosphorylated at Tyr418 in chick embryo fibroblasts expressing human wild-type Src.
This PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of Src (pTyr418).
a synthetic phosphopeptide corresponding to amino acids surrounding the pTyr418 phosphorylation site of human Src
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Martin, G.S. 2001. Nat. Rev. Mol. Cell Biol.2, 467.Reissig, D., et al. 2001. J. Cancer Res. Clin. Oncol.127, 226.Bjorge, J.D., et al. 2000. Oncogene19, 5620.Irby, R.B. and Yeatman, T.J. 2001. Oncogene19, 5636.Miermont, A.M., et al. 2000. J. Immunol. Methods246, 203.
Does not cross-react with non-phosphorylated Src, or with unrelated phosphorylation sites. The sequence of the immunogen is conserved in human (Tyr418), mouse (Tyr423), rat (Tyr418), chicken (Tyr416), and c-Src (Tyr416). Variables associated with assay conditions will dictate the optimal working dilution.
100 µg in Plastic ampoule
Lyophilized from 2X PBS, PEG, sucrose.
Reconstitute in 1 ml sterile dH2O (15 min, room temperature). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
Toxicity: Standard Handling (A)