Analysis Note

ControlMCF7 cell lyasate

Application

Research CategoryCell Structure

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Epithelial Specific Antigen in A431 cells.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Epithelial Specific Antigen in human prostate adenocarcinoma cells.

Anti-Epithelial Specific Antigen Antibody, clone VU-1D9 is a highly specific mouse monoclonal antibody, that targets Epithelial Cell Marker & has been tested in western blotting, ICC & IHC.

Research Sub CategoryECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Epithelial specific antigen, also known as epithelial cell adhesion molecule (Ep-CAM) and epithelial glycoprotein (EGP), may act as an interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Epithelial specific antigen plays a role in embryonic stem cells proliferation and differentiation, and up-regulates the expression of FABP5, MYC, and cyclins A and E. Epithelial specific antigen is highly and selectively expressed by undifferentiated embryonic stem cells (ESCs). Protein levels rapidly diminish as soon as ESCs differentiate. Also expressed in most epithelial cell membranes and on the surface of adenocarcinoma.

Immunogen

NCI-H69 whole cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in MCF7 cell lyate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Epithelial Specific Antigen in 10 µg of MCF7 cell lyate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~40 kDa observed. The calculated molecular weight of this protein is 35 kDa; however, this protein will run higher due to glycosylation.